مقاله ۲۰۱۳: تاثیر کفیر بر روی سلول های سرطانی
Induction of apoptosis of gastric cancer cells SGC7901 in vitro by a cell-free fraction of Tibetan kefir
Abstract
In this study, the anti-proliferative effect of cell-free fraction of Tibetan kefir on human gastric cancer cell line SGC7901 in vitro was investigated. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed a dose-dependent antiproliferative effect, and when the concentration of Tibetan kefir cell-free fraction was more than 8 mg mL−1, a distinct anti-proliferation of SGC7901 cells was observed. Flow cytometric analysis was carried out to detect cell cycle arrest and apoptosis. SGC7901 cells treated with a cell-free fraction of Tibetan kefir were inhibited in the G1/S phase, and both early and late apoptosis could be detected. To further investigate the mechanism of action of cell-free fraction of Tibetan kefir on apoptosis of SGC7901 cells, expression of human bcl-2 and bax genes were measured by qPCR. Apoptosis induced by Tibetan kefir was effected through up-regulation of bax and down-regulation of bcl-2.
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مقاله ۲۰۱۳,
تاثیر کفیر بر روی سلول های سرطانی
PF TEK چیست؟
INTRODUCTION
The PF TEK is basically a brown rice
method with an improved formula by using
vermiculite as a base and adding pulverized
brown rice. The secret is in the vermiculite.
When mycelium is cultured in just grain, the
mycelium turns into a mass with little air
space. But when grown with vermiculite,
the mycelial threads stretch across space.
The important thing about the PF TEK, is
that it copies nature. Instead of the usual
cloning of mushroom tissue and growing
mushrooms from that, a mass spore
inoculation is employed directly to the
fruiting substrate. That way, the genotype
remains complete. Senescence (mutating
and ceased fruiting) is no longer a problem.
The spores insure a never ending
succession of fungus, with all the power of
the spores reproductive ability intact.
قارچ ایران زمین
09128431824
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مقاله ۲۰۱۲: مدیریت بیمارگر های قارچ خوراکی از طریق مواد موثره گیاهی
برای مشاهده متن کامل به ادامه مطلب مراجعه نمایید (مخصوص اعضای ویژه)
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مقاله ۲۰۱۲,
مدیریت بیمارگر های قارچ خوراکی از طریق مواد موثره
مقاله ۲۰۱۲: بهبود فعالیت کاتالایتیکی، مقاومت دماویی، و تجزیه رنگ توسط لاکیز جداشده از قارچ صدفی با استفاده از ژل هیدروفوب
Improvement of Catalytic Efficiency, Thermo-stability and Dye Decolorization Capability of Pleurotus ostreatus IBL-02 laccase by Hydrophobic Sol Gel Entrapment.
ABSTRACT:
BACKGROUND: In serious consideration of the worldwide environmental issues associated with the extensive use of the textile dyes and effluents generated thereof, the scientists across the world are in search for potential treatment technologies for their treatment. In such scenario the ligninolytic enzymes provide a potential alternative because they are cost effective, eco-friendly and can be applied to wide range of dye containing industrial effluents.
RESULTS:
Laccase produced from Pleurotus ostreatus IBL-02 during decolorization of the reactive textile dye Drimarene brilliant red K-4BL (DBR K-4BL) was purified and immobilized by hydrophobic gel entrapment. The crude laccase was 4.2-fold purified with specific activity of 573.52 U/mg after passing through the DEAE-Sepharose ion exchange and Sephadex-G-100 chromatography columns. P. ostreatus IBL-02 laccase was found to be a homogenous monomeric protein as evident by single band corresponding to 67 kDa on native and sodium dodesylsulfate polyacrylamide gel electrophoresis (PAGE). The laccase was immobilized by entrapment in Sol--gel matrix of trimethoxysilane (T) and proplytetramethoxysilane (P) prepared using different T:P molar ratios. The free and immobilized laccases were compared to investigate the effect of immobilization on catalytic efficiency and thermo-stability features. Laccase immobilized in the Sol--gel of 1:5 T:P ratio was optimally active and thermo-stable fraction at pH 5, 60[degree sign]C with half-life of 3 h and 50 min. Laccases immobilized in 1:2 and 1:5 T:P ratio gels had significantly higher Km (83 and100mM) and Vmax (1000 and 1111 mM/mg) values as compared to free laccase. After 5 h reaction time varying decolorization percentages with a maximum of 100% were achieved for different dyes and effluents.
CONCLUSIONS:
In summary, P. ostreatus IBL-02 laccase was immobilized by entrapping in a Sol--gel matrix with an objective to enhance its catalytic and stability properties. Sol--gel entrapped laccase presented potential efficiency as a biocatalyst when applied for decolorization of different dyes and effluents. The main benefits of the Sol--gel matrix immobilization processes are the eco-friendly approach, chemical free and energy saving reaction conditions
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مقاله ۲۰۱۲,
بهبود فعالیت کاتالایتیکی,
مقاومت دماویی,
و تجزیه رنگ توسط لاکیز جداشده از قارچ صدفی با است
